epsin1 ecfp Search Results


epsin1 ecfp  (Addgene inc)
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Addgene inc epsin1 ecfp
Epsin1 Ecfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
epsin1 ecfp - by Bioz Stars, 2026-02
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synapto iatpsnfr2 mirfp670nano3  (Addgene inc)
92
Addgene inc synapto iatpsnfr2 mirfp670nano3
Fig. 1. PGK1 activation restores synaptic function under hypometabolic conditions. (A) Assay schematic: Cultured primary hippocampal neurons expressing vGlutI-pH or <t>synapto-iATPSnFR2-miRFP670nano3</t> were incubated in imaging medium of interest for 5 min before 10 bouts of AP firing (100 APs at 10 Hz, highlighted as black bars in the traces) delivered every minute. Created using Biorender.com. (B) Ensemble average vGlutI-pH responses are normalized to the maximal sensor fluorescence revealed by perfusion with 50 mM NH4Cl. With sufficient fuel (5 mM glucose, teal), efficient SV recycling persists for all rounds of stimulation, but in low glucose (0.1 mM glucose, blue), SV recycling gradually slows, resulting in a net accumulation of fluorescence. (C) The ensemble average fraction of the fluorescence remaining 55 s after stimulation for each bout for high- and low-glucose conditions. Means ± SEM for the data shown in (B). 5 mM glucose, n = 5; 0.1 mM glucose, n = 23. *P < 0.05 and **P < 0.01, two-way analysis of variance (ANOVA). (D) Ensemble average vGlutI-pH fluorescence in neurons expressing PGK1-HALO (red) and Terazosin-treated (green) shows that synaptic endurance is restored compared to 0.1 mM glucose control (blue). (E) The remaining fluorescence 55 s after stimulation after each train is plotted. Means ± SEM. Control, n = 23; PGK1- HALO, n = 12. **P < 0.01 and ***P < 0.001, two-way ANOVA multiple comparisons to control. Terazosin, n = 10. ##P < 0.01 and ###P < 0.001, two-way ANOVA multiple comparisons to control. (F) PGK1 (magenta) and synapsin I/II (cyan) immunofluorescence shows that PGK1 is present in nerve terminals (white arrows). Scale bar, 6 μm.
Synapto Iatpsnfr2 Mirfp670nano3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/synapto iatpsnfr2 mirfp670nano3/product/Addgene inc
Average 92 stars, based on 1 article reviews
synapto iatpsnfr2 mirfp670nano3 - by Bioz Stars, 2026-02
92/100 stars
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Fig. 1. PGK1 activation restores synaptic function under hypometabolic conditions. (A) Assay schematic: Cultured primary hippocampal neurons expressing vGlutI-pH or synapto-iATPSnFR2-miRFP670nano3 were incubated in imaging medium of interest for 5 min before 10 bouts of AP firing (100 APs at 10 Hz, highlighted as black bars in the traces) delivered every minute. Created using Biorender.com. (B) Ensemble average vGlutI-pH responses are normalized to the maximal sensor fluorescence revealed by perfusion with 50 mM NH4Cl. With sufficient fuel (5 mM glucose, teal), efficient SV recycling persists for all rounds of stimulation, but in low glucose (0.1 mM glucose, blue), SV recycling gradually slows, resulting in a net accumulation of fluorescence. (C) The ensemble average fraction of the fluorescence remaining 55 s after stimulation for each bout for high- and low-glucose conditions. Means ± SEM for the data shown in (B). 5 mM glucose, n = 5; 0.1 mM glucose, n = 23. *P < 0.05 and **P < 0.01, two-way analysis of variance (ANOVA). (D) Ensemble average vGlutI-pH fluorescence in neurons expressing PGK1-HALO (red) and Terazosin-treated (green) shows that synaptic endurance is restored compared to 0.1 mM glucose control (blue). (E) The remaining fluorescence 55 s after stimulation after each train is plotted. Means ± SEM. Control, n = 23; PGK1- HALO, n = 12. **P < 0.01 and ***P < 0.001, two-way ANOVA multiple comparisons to control. Terazosin, n = 10. ##P < 0.01 and ###P < 0.001, two-way ANOVA multiple comparisons to control. (F) PGK1 (magenta) and synapsin I/II (cyan) immunofluorescence shows that PGK1 is present in nerve terminals (white arrows). Scale bar, 6 μm.

Journal: Science advances

Article Title: Phosphoglycerate kinase is a central leverage point in Parkinson's disease-driven neuronal metabolic deficits.

doi: 10.1126/sciadv.adn6016

Figure Lengend Snippet: Fig. 1. PGK1 activation restores synaptic function under hypometabolic conditions. (A) Assay schematic: Cultured primary hippocampal neurons expressing vGlutI-pH or synapto-iATPSnFR2-miRFP670nano3 were incubated in imaging medium of interest for 5 min before 10 bouts of AP firing (100 APs at 10 Hz, highlighted as black bars in the traces) delivered every minute. Created using Biorender.com. (B) Ensemble average vGlutI-pH responses are normalized to the maximal sensor fluorescence revealed by perfusion with 50 mM NH4Cl. With sufficient fuel (5 mM glucose, teal), efficient SV recycling persists for all rounds of stimulation, but in low glucose (0.1 mM glucose, blue), SV recycling gradually slows, resulting in a net accumulation of fluorescence. (C) The ensemble average fraction of the fluorescence remaining 55 s after stimulation for each bout for high- and low-glucose conditions. Means ± SEM for the data shown in (B). 5 mM glucose, n = 5; 0.1 mM glucose, n = 23. *P < 0.05 and **P < 0.01, two-way analysis of variance (ANOVA). (D) Ensemble average vGlutI-pH fluorescence in neurons expressing PGK1-HALO (red) and Terazosin-treated (green) shows that synaptic endurance is restored compared to 0.1 mM glucose control (blue). (E) The remaining fluorescence 55 s after stimulation after each train is plotted. Means ± SEM. Control, n = 23; PGK1- HALO, n = 12. **P < 0.01 and ***P < 0.001, two-way ANOVA multiple comparisons to control. Terazosin, n = 10. ##P < 0.01 and ###P < 0.001, two-way ANOVA multiple comparisons to control. (F) PGK1 (magenta) and synapsin I/II (cyan) immunofluorescence shows that PGK1 is present in nerve terminals (white arrows). Scale bar, 6 μm.

Article Snippet: The following previously published DNA constructs were used: vGLUT1- pH (38), DJ- 1 (a gift from M. Cookson, Addgene, plasmid #29347, RRID: Addgene_29347, http://n2t.net/addgene:29347), synapto- iATPSnFR2- miRFP670nano3 (Addgene, plasmid #209730, RRID: Addgene_209730, http://n2t.net/addgene:209730), and synaptocpsfGFP- miRFP670nano3 (Addgene, plasmid #214926, RRID: Addgene_214926, http://n2t.net/addgene:214926).

Techniques: Activation Assay, Cell Culture, Expressing, Incubation, Imaging, Fluorescence, Control, Immunofluorescence